circRNA basic information
circBase ID: hsa_circ_0001346
Name: hsa_circ_RNF13
Synonym: circRNF13 / circ-0001346
Host Gene: RNF13
Genomic location(hg19): chr3:149563797-149639014:+
Genomic location(hg38): chr3:149846010-149921227:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009831
MONDO name: malignant pancreatic neoplasm
Disease details: pancreatic cancer / PC
Disease DO ID:
 1793
Disease MeSH ID:
 -
Disease NCIt ID:
 C9005
Disease ICD11 ID:
 542147910
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

pancreatic cancer tissues; adjacent normal tissues
Cell lines:

MIA PaCa-2; SW-1990; PANC-1; AsPC-1; BxPC-3; HPDE6-c7; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PDK3, HIF-1alpha, EIF4A3
Associated microRNA: miR-654-3p
Biological function: promotes proliferation; promotes angiogenesis; promotes migration and invasion; promotes metastasis; promotes glycolysis; inhibits oxidative phosphorylation
Molecular mechanism: Hypoxia/HIF-1alpha and EIF4A3 promote circRNF13 biogenesis; circRNF13 acts as a ceRNA sponge for miR-654-3p to increase PDK3 expression
Biological pathway or process:

proliferation (promotes); angiogenesis (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); oxidative phosphorylation (inhibits); ceRNA regulation (promotes)
Detected method:
Q
H
S
Validation methods:

RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Tube Formation Assay; Western Blot; ChIP / ChIP-seq; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Survival Analysis
Clinical significance:

circRNF13 expression was higher in PC tissues and patients with higher circRNF13 expression had worse overall survival (OS); circRNF13 could serve as a potential diagnostic and prognostic factor for PC patients
Description:

In pancreatic cancer, circRNF13 (hsa_circ_0001346) is up-regulated (including under hypoxia), promotes proliferation, angiogenesis, invasion/metastasis and glycolysis, and is associated with poor overall survival. Mechanistically, its biogenesis is promoted by HIF-1alpha and EIF4A3, and it acts as a cytoplasmic ceRNA that sponges miR-654-3p to upregulate PDK3.
Confidence score:

0.8941
Other information
Title:

Hypoxia-induced circRNF13 promotes the progression and glycolysis of pancreatic cancer.

Journal: Experimental & molecular medicine
Published: 2022
PubMed ID: 36369467
Study type:

combined biological and clinical study
Data availability: -
Code availability: -