| Expression pattern: |
UP |
| Associated gene: |
TRA2B, ELAVL1, 3'UTR of TRA2B, NF-kappaB2, NF-kappaB p65, p-NF-kappaB p65 |
| Associated microRNA: |
miR-432-5p |
| Biological function: |
Promotes NSCLC cell migration and invasion in vitro, facilitates tumor growth in vivo, and enhances migratory and invasive capacities of recipient cells through exosomal delivery. |
| Molecular mechanism: |
Exosomal circ_0008803 acts through dual mechanisms: it sponges miR-432-5p to relieve repression of TRA2B, and binds ELAVL1 to promote ELAVL1 cytoplasmic translocation and stabilize TRA2B mRNA via 3'UTR interaction, thereby activating the NF-kappaB pathway. |
| Biological pathway or process: |
NF-kappaB (promotes); migration (promotes); invasion (promotes); metastasis (promotes); proliferation (promotes); ceRNA regulation (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Elevated serum exosomal circ_0008803 levels correlated with advanced NSCLC stages and distant metastasis, and serum exosomal circ_0008803 showed diagnostic potential for NSCLC. |
| Description: |
circ_0008803 is an exosome-enriched RNF10-derived circRNA up-regulated in NSCLC serum exosomes and lung cancer cells. It promotes migration, invasion, metastasis-related phenotypes, and tumor growth by sponging miR-432-5p and binding ELAVL1 to increase TRA2B expression and activate NF-kappaB signaling. Serum exosomal circ_0008803 also shows diagnostic potential and correlates with metastasis and tumor stage. |
| Confidence score: |
0.8791 |