| Expression pattern: |
UP |
| Associated gene: |
COL5A2 |
| Associated microRNA: |
miR-139-5p, miR-7-5p, miR-221-3p, miR-7 |
| Biological function: |
hsa_circ_0000253 promotes osteosarcoma cell proliferation and migration and may contribute to EMT and OS progression. |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0000253 competitively binds miR-7 and regulates COL5A2 expression; hsa_circ_0000253 knockdown increases miR-7 and decreases COL5A2, suppressing proliferation and migration. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); EMT (promotes); immune regulation (other) |
| Detected method: |
M
|
| Validation methods: |
Bioinformatics Analysis; Transfection; RT-qPCR; MTT; Transwell Assay; Wound Healing Assay |
| Clinical significance: |
The hsa_circ_0000253/miR-7/COL5A2 axis is proposed as a critical driver of OS malignancy and a potential therapeutic target, but no direct circRNA-specific prognostic or diagnostic association was reported. |
| Description: |
This study identifies hsa_circ_0000253 as an up-regulated circRNA in osteosarcoma and prioritizes it as the central circRNA in a ceRNA network. Functional knockdown experiments in U2OS cells indicate that hsa_circ_0000253 promotes OS cell proliferation and migration, likely through the hsa_circ_0000253/miR-7/COL5A2 axis. The evidence for differential expression is based on GEO transcriptome/circRNA profile analysis rather than RT-qPCR validation of disease up-regulation. |
| Confidence score: |
0.4546 |