| Expression pattern: |
DN |
| Associated gene: |
TFRC mRNA, TFRC, pre-ZMYM4 |
| Associated microRNA: |
hsa-miR-576-5p |
| Biological function: |
hsa_circ_0011536 inhibits proliferation and induces ferroptosis in NSCLC cells. |
| Molecular mechanism: |
m6A modification enhances the back-splicing of pre-ZMYM4 to increase hsa_circ_0011536 expression; hsa_circ_0011536 acts as a sponge for hsa-miR-576-5p to elevate TFRC expression and induce ferroptosis. |
| Biological pathway or process: |
ferroptosis (promotes); proliferation (inhibits); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; MeRIP / MeRIP-seq; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
These results might provide novel therapeutic targets for NSCLC treatment. |
| Description: |
hsa_circ_0011536 is downregulated in NSCLC tissues and cell lines. m6A modification promotes its production from pre-ZMYM4, and hsa_circ_0011536 functions as a hsa-miR-576-5p sponge to increase TFRC expression. Functionally, hsa_circ_0011536 suppresses NSCLC cell proliferation and promotes ferroptosis, suggesting potential therapeutic relevance. |
| Confidence score: |
0.6815 |