| Expression pattern: |
UP |
| Associated gene: |
PD-L1 / programmed death ligand-1, Ago2 |
| Associated microRNA: |
miR-488-3p |
| Biological function: |
Silencing circ_0000372 represses gastric cancer cell proliferation, invasion and immune escape, reduces tumor formation and tumor weight in vivo, and increases CD8+ T cell percentage and IFN-gamma while reducing PD-L1 and IL-10. |
| Molecular mechanism: |
circ_0000372 acts as a miR-488-3p sponge to positively regulate PD-L1 expression, thereby promoting gastric cancer proliferation, invasion and immune escape. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Flow Cytometry（Non-apoptosis/cycle）; ELISA; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0000372 expression indicated poor prognosis in gastric cancer and was linked to TNM stage and lymph node metastasis. |
| Description: |
circ_0000372 is up-regulated in gastric cancer tissues and cells, and high expression is associated with poor prognosis and advanced clinicopathological features. Functionally, circ_0000372 promotes GC cell proliferation, invasion and immune escape. Mechanistically, it sponges miR-488-3p to positively regulate PD-L1, while circ_0000372 knockdown suppresses tumor growth and immune escape in vivo. |
| Confidence score: |
0.7976 |