| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-335 |
| Biological function: |
circ-EIF4G3 promotes gastric cancer cell proliferation, invasion and migration; knockdown of circ-EIF4G3 inhibits these malignant behaviors. |
| Molecular mechanism: |
circ-EIF4G3 acts as a miR-335 sponge in the cytoplasm, thereby promoting proliferation, invasion and migration of gastric cancer cells. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Microarray; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Increased circ-EIF4G3 expression was associated with higher TNM stage and lymphatic metastasis, and showed diagnostic value for distinguishing GC tissues from adjacent non-cancerous tissues with AUC 0.7158. |
| Description: |
circ-EIF4G3 is up-regulated in gastric cancer tissues and GC cell lines, and its higher expression is associated with TNM stage and lymphatic metastasis. Functionally, circ-EIF4G3 promotes GC cell proliferation, invasion and migration. Mechanistically, it is mainly cytoplasmic and acts as a sponge for miR-335. |
| Confidence score: |
0.8256 |