| Expression pattern: |
UN |
| Associated gene: |
SIRT1, Ago2 |
| Associated microRNA: |
miR-145 |
| Biological function: |
Promotes therapeutic neovascularization and angiogenesis, improves endothelial cell viability, proliferation, migration and tube formation, and attenuates hypoxia-induced apoptosis. |
| Molecular mechanism: |
Exosomal hsa_circ_0093884 acts as a cytoplasmic miR-145 sponge and ceRNA, relieving miR-145-mediated repression of SIRT1 to promote therapeutic neovascularization. |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Tube Formation Assay; TUNEL; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Promising therapeutic target for exosome-mediated transplantation in ischemic disease repair and therapeutic neovascularization. |
| Description: |
This study identifies hsa_circ_0093884 as a SIRT1-derived exosomal circRNA enriched in endothelial progenitor cell-derived exosomes. Exosomal hsa_circ_0093884 promotes therapeutic neovascularization by enhancing endothelial proliferation, migration and angiogenesis while reducing hypoxia-induced apoptosis. Mechanistically, it acts as a cytoplasmic sponge for miR-145, relieving repression of SIRT1 through the hsa_circ_0093884/miR-145/SIRT1 axis. |
| Confidence score: |
0.8165 |