| Expression pattern: |
UP |
| Associated gene: |
RUNX1, KEAP1, AGO2, PI3K/AKT/mTOR pathway |
| Associated microRNA: |
miR-331-3p |
| Biological function: |
circ_0049271 promotes LPS-induced HK-2 cell injury, oxidative stress, apoptosis, and inflammation; circ_0049271 silencing alleviates these injury phenotypes and enhances EdU-positive cell rate. |
| Molecular mechanism: |
circ_0049271 acts as a ceRNA by sponging miR-331-3p, thereby positively regulating RUNX1 expression and activating the PI3K/AKT/mTOR pathway in LPS-induced HK-2 cells. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); apoptosis (promotes); inflammation (promotes); proliferation (inhibits); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0049271 may be a potential molecular target for SAKI treatment. |
| Description: |
circ_0049271 is up-regulated in LPS-induced HK-2 cells, an in vitro model of sepsis-associated AKI. Functionally, circ_0049271 promotes oxidative stress, apoptosis and inflammatory cytokine secretion, while its knockdown alleviates these injury phenotypes. Mechanistically, circ_0049271 sponges miR-331-3p to increase RUNX1 expression and activate the PI3K/AKT/mTOR pathway. |
| Confidence score: |
0.8081 |