circRNA basic information
circBase ID: hsa_circ_0000977
Name: hsa_circ_NOL10
Synonym: hsa_circ_001918
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

cancerous lung tissue; paracancerous lung tissue; subcutaneous tumors; lung tissues
Cell lines:

A549; H1299; H226; H460; H661; SK-MES-1; BEAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: SCML1, ESRP1, Pre-NOL10, HN1, HN2, HN3, HN5, HN6, HN8, HN9, HN10, ubiquitin
Associated microRNA: -
Biological function: inhibits lung cancer development by inhibiting cell proliferation and cell cycle progression and promoting apoptosis
Molecular mechanism: circNOL10 binds SCML1 and inhibits SCML1 ubiquitination, increasing SCML1 protein; SCML1 transcriptionally activates the humanin (HN) polypeptide family (e.g., HN2, HN8), altering mitochondrial function (ROS/MMP/ATP) and downstream tumor signaling molecules (e.g., p53, CHEK2, CDC25A, CCND1, CDK2, Rb, E2F1).
Biological pathway or process:

proliferation (inhibits); cell cycle (inhibits); apoptosis (promotes); mitochondrial function (other pathway/process); other pathway/process (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; FISH / smFISH; RNA Pull-Down; Western Blot; Luciferase Reporter Assay; Transfection; Co-IP; ChIP / ChIP-seq; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Flow Cytometry（Non-apoptosis/cycle）; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

circNOL10 expression is significantly lower in lung cancer tissues and differs by histological type and differentiation degree.
Description:

circNOL10 is down-regulated in lung cancer and functions as a tumor suppressor. It directly interacts with SCML1 to inhibit SCML1 ubiquitination, increases SCML1 expression, and enhances SCML1-mediated transcription of the humanin polypeptide family (e.g., HN2/HN8), leading to altered mitochondrial function and reduced proliferation/cell-cycle progression with increased apoptosis.
Confidence score:

0.8096
Other information
Title:

Circular RNA circNOL10 Inhibits Lung Cancer Development by Promoting SCLM1-Mediated Transcriptional Regulation of the Humanin Polypeptide Family.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published: 2019
PubMed ID: 30693177
Study type:

combined biological and clinical study
Data availability: Table S1; Table S2; Table S3; Supporting Information
Code availability: -