circRNA basic information
circBase ID: hsa_circ_0001649
Name: hsa_circ_SHPRH
Synonym: circSHPRH
Host Gene: SHPRH
Genomic location(hg19): chr6:146209155-146216113:-
Genomic location(hg38): chr6:145888019-145894977:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0010150
MONDO name: head and neck squamous cell carcinoma
Disease details: head and neck squamous cell carcinoma
Disease DO ID:
 5520
Disease MeSH ID:
 C535575
Disease NCIt ID:
 C34447
Disease ICD11 ID:
 -
Disease OMIM ID:
 275355
Species: Human
Species details: Homo sapiens
Tissue specimen:

HNSCC tissues; adjacent non-tumor tissues; xenograft tumors
Cell lines:

SCC-9; SCC-15; SCC-25; CAL-27; HOK
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PI3K, AKT, mTOR
Associated microRNA: -
Biological function: CircSHPRH acts as a tumor suppressor in HNSCC by inhibiting cell proliferation, migration, invasion, and tumor growth, and by promoting apoptosis.
Molecular mechanism: CircSHPRH suppresses the PI3K/AKT/mTOR signaling pathway, reducing phosphorylation of PI3K, AKT, and mTOR; activation of PI3K by 740Y-P reverses circSHPRH-mediated phenotypes.
Biological pathway or process:

PI3K/AKT/mTOR (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); EMT (inhibits); metastasis (inhibits)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); TUNEL; Cohort Study; Survival Analysis
Clinical significance:

Low circSHPRH expression was associated with shorter overall survival and histological grade; circSHPRH may serve as a potential biomarker for HNSCC diagnosis, prognosis, and therapeutic response prediction.
Description:

CircSHPRH is a human circRNA derived from SHPRH and is significantly downregulated in HNSCC tissues and cell lines. In this study, circSHPRH overexpression suppressed proliferation, migration, invasion, EMT-related marker changes, and xenograft tumor growth while promoting apoptosis. Mechanistically, circSHPRH acted as a tumor suppressor at least partly by inhibiting PI3K/AKT/mTOR signaling, and higher expression was associated with better overall survival.
Confidence score:

0.8169
Other information
Title:

CircSHPRH inhibits malignancy progression of head and neck squamous cell carcinoma by regulating PI3K/AKT/mTOR signaling pathway.

Journal: Functional & integrative genomics
Published: 2025
PubMed ID: 40913157
Study type:

combined biological and clinical study
Data availability: -
Code availability: -