| Expression pattern: |
UN |
| Associated gene: |
H3 |
| Associated microRNA: |
- |
| Biological function: |
Limits DNA damage and supports MLL-r leukemia cell proliferation, survival, differentiation blockade and self-renewal; silencing induces DDR, inhibits proliferation, promotes apoptosis and overcomes differentiation blockade. |
| Molecular mechanism: |
Chromatin-associated cacircRNA that promotes DDR and NHEJ-mediated DNA repair in MLL-r leukemia cells; detailed interacting proteins were not defined for circABR(2,15) in the provided text. |
| Biological pathway or process: |
other pathway/process (promotes); proliferation (promotes); apoptosis (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; Colony Formation Assay; Western Blot; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circABR(2,15) is a human chromatin-associated circRNA functionally investigated in MLL-r leukemia cells. Its depletion increases DNA damage and produces anti-leukemic phenotypes including reduced proliferation, increased apoptosis, differentiation induction and impaired self-renewal, suggesting an oncogenic role in MLL-r leukemia. |
| Confidence score: |
0.6988 |