| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, PTBP3, HIF-1alpha 5′UTR, HIF-1alpha, VEGFA |
| Associated microRNA: |
- |
| Biological function: |
Promotes glioma cell proliferation, migration, invasion, tumor growth, angiogenesis, vascular endothelial cell proliferation, vascular sprouting, and tube formation. |
| Molecular mechanism: |
EIF4A3 binds to the flanking intronic sequences of circCRIM1 precursor RNA and promotes circCRIM1 circularization; circCRIM1 acts as a molecular scaffold that binds PTBP3, strengthens PTBP3 association with the HIF-1alpha 5′UTR, enhances HIF-1alpha translation, and upregulates VEGFA. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); HIF-1 signaling (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Tube Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
Elevated levels significantly correlate with tumor growth, metastasis, and poor prognosis, suggesting potential as a biomarker for cancer diagnosis and treatment response; inhibition of circCRIM1 may represent a promising anti-GBM therapeutic approach. |
| Description: |
In this study, circCRIM1 is a human CRIM1-derived circRNA upregulated in glioma cells and functions as an oncogenic circRNA in glioblastoma/glioma. It promotes malignant progression and angiogenesis by scaffolding PTBP3 to the HIF-1alpha 5′UTR, enhancing HIF-1alpha protein translation and VEGFA expression; EIF4A3 also promotes its circularization under hypoxic conditions. |
| Confidence score: |
0.7982 |