circRNA basic information
circBase ID: hsa_circ_0049101
Name: hsa_circ_MUC16
Synonym: circMUC16
Host Gene: MUC16
Genomic location(hg19): chr19:8987209-8993545:-
Genomic location(hg38): chr19:8876533-8882869:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

clinical blood samples; plasma; peripheral blood
Cell lines:

NIH:OVCAR-3; SKOV3; IOSE-80; NIH:OVCAR3
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: Serves as an ovarian cancer RNA biomarker for liquid biopsy detection; expression increases in OC cell lines and clinical blood samples and helps distinguish early and advanced stages of OC.
Molecular mechanism: No disease functional mechanism was investigated; the study used MIRACLE SlipChip with BstNI-directed circRNA back-splice junction identification, RCA amplification, and CRISPR/Cas12a fluorescence detection for biomarker quantification.
Biological pathway or process:

not specified
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; ROC Analysis
Clinical significance:

hsa_circ_0049101 is a diagnostic RNA biomarker for ovarian cancer liquid biopsy, with AUC 0.985 for all-stage OC and AUC 0.969 for early-stage OC in the MIRACLE SlipChip assay.
Description:

This study evaluated hsa_circ_0049101/circMUC16 as an ovarian cancer liquid biopsy biomarker rather than as a mechanistic regulator. Its expression was higher in OC cell lines and clinical blood samples, increased with disease stage, correlated strongly with RT-qPCR measurements, and showed high diagnostic performance for both all-stage and early-stage OC.
Confidence score:

0.5261
Other information
Title:

Modular RCA-CRISPR/Cas12a amplification on a multi-volume SlipChip for ultrafast, single-copy quantification of circRNA and miRNA in ovarian cancer.

Journal: Lab on a chip
Published: 2025
PubMed ID: 40878190
Study type:

combined technological and clinical study
Data availability: Supplementary information; DOI: https://doi.org/10.1039/D5LC00585J; Tables S1-S3; Fig. S1-S16
Code availability: -