| Expression pattern: |
UP |
| Associated gene: |
cullin 4A (CUL4A) |
| Associated microRNA: |
miR-665 |
| Biological function: |
circSNTB2 inhibits NP cell proliferation and promotes apoptosis; knockdown of circSNTB2 promotes NP cell proliferation, inhibits apoptosis, alleviates thermo-mechanical pain and NP injury, and delays LDH progression in rats. |
| Molecular mechanism: |
circSNTB2 functions as a ceRNA that binds miR-665, thereby regulating the downstream target gene CUL4A and affecting NP cell proliferation and apoptosis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (inhibits); apoptosis (promotes); inflammation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; TUNEL; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; H&E Staining; ELISA |
| Clinical significance: |
Knockdown of circSNTB2 may be a potential target to protect NP cells from damage and provides a potential idea for targeted therapy of LDH. |
| Description: |
circSNTB2 is up-regulated in NP tissues from LDH patients and in TNF-alpha-treated NP cells. It promotes LDH-related NP cell dysfunction by acting through the miR-665/CUL4A axis, inhibiting NP cell proliferation and promoting apoptosis, while circSNTB2 knockdown alleviates LDH progression in a rat model. |
| Confidence score: |
0.7053 |