| Expression pattern: |
DN |
| Associated gene: |
CDK1, p21 |
| Associated microRNA: |
miR-605 |
| Biological function: |
circDDX17 acts as a tumour suppressor in breast cancer; overexpression inhibits cell proliferation and colony formation, induces G0/G1 cell cycle arrest, and promotes apoptosis. |
| Molecular mechanism: |
circDDX17 functions as a competing endogenous RNA and sponge for miR-605, suppressing proliferation through regulation of cell cycle-related factors CDK1 and p21. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
Low circDDX17 expression was associated with tumour grade, lymph node infiltration, advanced TNM stage, and poor overall survival; it may serve as a potential biomarker and therapeutic target for breast cancer. |
| Description: |
circDDX17 is downregulated in breast cancer tissues and cell lines and acts as a tumour suppressor. It sponges miR-605 to regulate cell cycle-related proteins CDK1 and p21, thereby inhibiting proliferation and colony formation, inducing cell cycle arrest, and promoting apoptosis. Its reduced expression is associated with aggressive clinicopathological features, suggesting biomarker and therapeutic potential. |
| Confidence score: |
0.6829 |