circRNA basic information
circBase ID: hsa_circ_0099682
Name: hsa_circ_ANKS1B
Synonym: hsa_ circ_0099682
Host Gene: ANKS1B
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005266
MONDO name: diabetic retinopathy
Disease details: diabetic retinopathy
Disease DO ID:
 8947
Disease MeSH ID:
 D003930
Disease NCIt ID:
 C34538
Disease ICD11 ID:
 1006882070
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

retinal tissues
Cell lines:

HRMECs
In vivo animal model:

other disease animal model
circRNA-disease information
Expression pattern:
UP
Associated gene: HNRNPU, Ago2, VEGFA, Occludin, PTEN
Associated microRNA: miR-125b-5p
Biological function: Accelerates DR progression; promotes pathological neoangiogenesis, mitochondrial dysfunction, oxidative stress, inflammation, apoptosis, and endothelial injury under high-glucose conditions; knockdown improves retinal structural integrity and suppresses tube-forming ability.
Molecular mechanism: ceRNA mechanism: hsa_circ_0099682 sponges miR-125b-5p, relieves miR-125b-5p-mediated repression of HNRNPU, increases HNRNPU expression, and thereby drives mitochondrial dysfunction, inflammatory responses, oxidative stress, and angiogenesis-related protein changes.
Biological pathway or process:

ceRNA regulation (promotes); angiogenesis (promotes); mitochondrial function (inhibits); inflammation (promotes); apoptosis (promotes); proliferation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Transfection; CCK8; Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry; ELISA; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IF (Immunofluorescence)
Clinical significance:

-
Description:

hsa_circ_0099682 is a pro-pathogenic circRNA in diabetic retinopathy models. It acts as a cytoplasmic ceRNA that sponges miR-125b-5p, derepresses HNRNPU, and promotes mitochondrial dysfunction, inflammation, oxidative stress, apoptosis, and pathological neoangiogenesis. Silencing hsa_circ_0099682 protects HG-injured HRMECs and improves retinal pathology in STZ-induced DR rats.
Confidence score:

0.7924
Other information
Title:

hsa_circ_0099682 exacerbates the development of diabetic retinopathy by promoting pathological neoangiogenesis via the miR-125b-5p/HNRNPU axis.

Journal: Journal of molecular histology
Published: 2025
PubMed ID: 40782251
Study type:

biological research
Data availability: Data is available from the corresponding author on request.
Code availability: -