| Expression pattern: |
UP |
| Associated gene: |
PDK2 |
| Associated microRNA: |
miR-1287 |
| Biological function: |
promotes proliferation and glycolysis; suppresses apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-1287 to upregulate PDK2. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression of circ_0091579 was associated with low overall survival and malignant clinicopathological characteristics (serum AFP, tumor size, TNM stage, metastasis) in patients with HCC. |
| Description: |
circ_0091579 is upregulated in HCC tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-1287 to increase PDK2 expression, thereby promoting proliferation and glycolysis while inhibiting apoptosis, and supports xenograft tumor growth. |
| Confidence score: |
0.8166 |