| Expression pattern: |
DN |
| Associated gene: |
TRAF4, Eg5 |
| Associated microRNA: |
- |
| Biological function: |
Suppresses breast cancer cell viability, promotes monastrol-induced cytotoxicity, and reverses monastrol resistance. |
| Molecular mechanism: |
circRNA-MTO1 interacts with TRAF4 and sequesters TRAF4 from activating Eg5 translation, thereby inhibiting Eg5 protein level at the post-transcriptional level. |
| Biological pathway or process: |
proliferation (inhibits); chemoresistance (inhibits); drug resistance (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Transfection; CCK8; IF (Immunofluorescence); Western Blot; RNA Pull-Down; circRIP; FISH / smFISH; Nuclear-Cytoplasmic Fractionation |
| Clinical significance: |
- |
| Description: |
circRNA-MTO1 is down-regulated in monastrol-resistant breast cancer cells. Its overexpression suppresses breast cancer cell viability, enhances monastrol cytotoxicity, and reverses monastrol resistance by binding TRAF4 and inhibiting TRAF4-mediated Eg5 protein translation. |
| Confidence score: |
0.6503 |