| Expression pattern: |
UN |
| Associated gene: |
IGF2BP2, TNS1 mRNA, AGO2 |
| Associated microRNA: |
- |
| Biological function: |
Promotes megakaryocyte proplatelet formation, platelet-like particle production, actin cytoskeleton reorganization, F-actin polymerization and assembly, and spreading and adhesion on extracellular matrix proteins. |
| Molecular mechanism: |
circFUT8 interacts with IGF2BP2 and enhances TNS1 mRNA stability in an m6A-dependent manner, thereby increasing TNS1 mRNA and protein levels and regulating actin cytoskeleton dynamics. |
| Biological pathway or process: |
proliferation (other); mRNA stability (promotes); m6A modification (other); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for thrombocytopenia and bleeding disorders. |
| Description: |
Human circFUT8 is upregulated during megakaryocyte maturation and is abundant in mature megakaryocytes and platelets. It promotes proplatelet formation by interacting with IGF2BP2 to stabilize TNS1 mRNA in an m6A-dependent manner, thereby supporting actin cytoskeleton organization and extracellular matrix spreading/adhesion. |
| Confidence score: |
0.8231 |