| Expression pattern: |
DN |
| Associated gene: |
STARD7 |
| Associated microRNA: |
miR-212-5p |
| Biological function: |
circEGLN1 acts as a protective factor that reduces CaOx/COM-induced renal tubular oxidative injury, cell death, ROS production, MDA levels, renal tubular injury, and CaOx crystal deposition. |
| Molecular mechanism: |
circEGLN1 functions through a ceRNA mechanism by sponging miR-212-5p, thereby increasing STARD7 expression and attenuating oxidative stress. |
| Biological pathway or process: |
apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circEGLN1 is significantly lower in stone-containing renal tissues than non-stone controls and is highlighted as a promising therapeutic target for preventing CaOx-induced kidney stone formation. |
| Description: |
circEGLN1 is a conserved EGLN1-derived circRNA that is down-regulated in COM-injured renal tubular cells and nephrolithiasis renal tissues. It protects against CaOx-induced renal oxidative injury and crystal deposition by acting as a miR-212-5p sponge to enhance STARD7 expression, suggesting therapeutic potential for kidney stone prevention. |
| Confidence score: |
0.8705 |