| Expression pattern: |
UP |
| Associated gene: |
YWHAZ |
| Associated microRNA: |
miR-433-3p |
| Biological function: |
Promotes BC cell proliferation/growth, angiogenesis, and stemness; inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-433-3p to regulate YWHAZ, leading to activation of PI3K/AKT signaling. |
| Biological pathway or process: |
proliferation (promotes); angiogenesis (promotes); stemness (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_0002496 expression in stage II and III tumors was augmented relative to stage I tumors. |
| Description: |
circ_0002496 is up-regulated in breast cancer tissues and cells and promotes malignant behaviors (proliferation/growth, angiogenesis, stemness) while inhibiting apoptosis. Mechanistically, it sponges miR-433-3p to increase YWHAZ and activate PI3K/AKT signaling, and its knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.7595 |