circRNA basic information
circBase ID: -
Name: hsa_circ_ASPH
Synonym: circASPH
Host Gene: ASPH
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumor tissues; adjacent nontumor liver tissue
Cell lines:

Huh-7; SMMC-7721; HepG2; MHCC97H; HCCLM3; THLE-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: DNMT3b, HAO2, 5mC
Associated microRNA: miR-370-3p
Biological function: promotes cell proliferation, migration, and invasion; promotes tumor growth in vivo
Molecular mechanism: acts as a miRNA sponge for miR-370-3p to regulate DNMT3b and 5mC, enhancing HAO2 promoter methylation and suppressing HAO2 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); m6A modification (not specified)
Detected method:
Q
H
M
Validation methods:

RT-qPCR; FISH / smFISH; Clinical Sample Validation; Survival Analysis; Cohort Study; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis; IHC (Immunohistochemistry); Western Blot; MeRIP / MeRIP-seq; Microarray
Clinical significance:

circASPH level was closely related to overall survival and was an independent prognostic factor in HCC patients
Description:

circASPH is up-regulated in HCC tissues/cell lines and promotes proliferation, migration, invasion and xenograft tumor growth. It acts as a cytoplasmic sponge for miR-370-3p, relieving repression of DNMT3b, increasing 5mC, enhancing HAO2 promoter methylation, and reducing HAO2 expression, thereby driving HCC progression and associating with worse overall survival.
Confidence score:

0.8308
Other information
Title:

CircASPH Promotes Hepatocellular Carcinoma Progression Through Methylation and Expression of HAO2.

Journal: Frontiers in oncology
Published: 2022
PubMed ID: 35795047
Study type:

combined biological and clinical study
Data availability: GSE28274; GSE125469; GSE55752; https://www.frontiersin.org/articles/10.3389/fonc.2022.911715/full#supplementary-material
Code availability: -