| Expression pattern: |
UP |
| Associated gene: |
E2F5 |
| Associated microRNA: |
miR-577 |
| Biological function: |
promotes proliferation, migration, invasion, glutaminolysis, and tumor growth of glioblastoma |
| Molecular mechanism: |
circFOXM1 acts as a miRNA sponge for miR-577 to positively regulate E2F5 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glutaminolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
circFOXM1 might be a biomarker for GBM treatment; high circFOXM1 expression was related to WHO grade and tumor size |
| Description: |
circFOXM1 is up-regulated in glioblastoma tissues and cell lines. Functionally, circFOXM1 promotes GBM proliferation, migration, invasion, glutaminolysis and xenograft tumor growth by sponging miR-577, thereby relieving repression of the downstream target E2F5. |
| Confidence score: |
0.8436 |