| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, EIF3I mRNA |
| Associated microRNA: |
- |
| Biological function: |
Enhances CRC cell malignancy by promoting proliferation, migration, invasion, and tumor growth; silencing hsa_circ_0113050 in exosomes reverses exosome-induced CRC cell malignancy and tumor growth. |
| Molecular mechanism: |
EIF4A3 binds to EIF3I mRNA, the linear precursor/host gene transcript of hsa_circ_0113050, and promotes hsa_circ_0113050 circularization/biogenesis; hsa_circ_0113050 is packaged into CRC-derived exosomes and contributes to CRC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0113050 expression is associated with advanced TNM stages, lymph node metastasis, and poor differentiation; it is highlighted as a promising therapeutic target and potential biomarker for noninvasive CRC diagnosis. |
| Description: |
This study identifies hsa_circ_0113050 as an up-regulated exosomal circRNA in colorectal cancer tissues and CRC-derived exosomes. EIF4A3 promotes hsa_circ_0113050 biogenesis through interaction with its linear precursor/host gene transcript EIF3I, and exosome-mediated hsa_circ_0113050 enhances CRC cell proliferation, migration, invasion, and tumor growth. Its high expression is associated with advanced clinicopathological features and may represent a therapeutic target or noninvasive biomarker candidate. |
| Confidence score: |
0.7785 |