| Expression pattern: |
UP |
| Associated gene: |
SLC7A11, GPX4, GCLC |
| Associated microRNA: |
miR-137 |
| Biological function: |
circPDSS1 functions as an oncogene in NSCLC cells; its silencing suppresses cell viability and colony formation and induces ferroptosis-mediated cell death. |
| Molecular mechanism: |
circPDSS1 acts as a miR-137 sponge and regulates the miR-137/SLC7A11/GPX4/GCLC axis to modulate ferroptosis in NSCLC cells. |
| Biological pathway or process: |
proliferation (promotes); ferroptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circPDSS1 may be used as a treatment target for NSCLC management. |
| Description: |
circPDSS1 is upregulated in NSCLC cell lines and acts as an oncogenic circRNA that supports NSCLC cell viability and colony formation. Its knockdown induces ferroptosis by relieving suppression of miR-137 and downregulating the SLC7A11/GPX4/GCLC axis, suggesting circPDSS1 as a potential therapeutic target for NSCLC. |
| Confidence score: |
0.7198 |