circRNA basic information
circBase ID: hsa_circ_0003270
Name: hsa_circ_GAPVD1
Synonym: circGAPVD1
Host Gene: GAPVD1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; paracancerous tissues; subcutaneous tumor tissue; mouse serum
Cell lines:

GES-1; HEK-293 T; HGC-27; AGS; MKN-45; MGC-803
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: STK4, GAPVD1-137aa, SMAD2/3, p-SMAD2/3
Associated microRNA: miR-4424
Biological function: Inhibits GC progression by suppressing migration, invasion, wound-healing capacity, proliferation, EMT, in vivo tumor growth, and inflammatory cytokine secretion, while promoting apoptosis.
Molecular mechanism: circGAPVD1 acts as a ceRNA sponge for miR-4424 to upregulate STK4, encodes the tumor-suppressive GAPVD1-137aa protein, and inhibits SMAD2/3 phosphorylation in the SMAD signaling pathway.
Biological pathway or process:

TGF-beta/SMAD (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); apoptosis (promotes); inflammation (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; ELISA; Western Blot; IF (Immunofluorescence); Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circGAPVD1 expression correlates with larger tumor size and advanced TNM stage; high circGAPVD1 expression predicts better overall survival and suggests diagnostic, prognostic, and therapeutic potential in GC.
Description:

circGAPVD1 is a human GAPVD1-derived circRNA downregulated in gastric cancer tissues and cell lines. It suppresses GC progression by sponging miR-4424 to regulate STK4, encoding the tumor-suppressive GAPVD1-137aa protein, and inhibiting SMAD signaling, thereby reducing proliferation, migration, invasion, EMT, inflammation, and tumor growth while promoting apoptosis. Clinically, higher circGAPVD1 expression is associated with better overall survival and lower tumor size/TNM stage.
Confidence score:

0.8557
Other information
Title:

circGAPVD1 inhibits the progression of gastric cancer through miR-4424/STK4 axis and encoding GAPVD1-137aa protein.

Journal: International journal of biological macromolecules
Published: 2025
PubMed ID: 40545108
Study type:

combined biological and clinical study
Data availability: GSE78092; GSE157013; GSE181769; Figs. 12345678; https://doi.org/10.1016/j.ijbiomac.2025.145408
Code availability: -