| Expression pattern: |
UP |
| Associated gene: |
Rab43 |
| Associated microRNA: |
miR-619-5p |
| Biological function: |
Promotes KGN cell proliferation, inhibits apoptosis, modulates growth and cell cycle progression of granulosa cells, and promotes PCOS progression. |
| Molecular mechanism: |
circ_BMPR2 functions as a ceRNA/miRNA sponge for miR-619-5p, thereby regulating Rab43 expression and affecting granulosa cell proliferation and apoptosis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_BMPR2 could be a prospective biomarker for the early diagnosis of PCOS. |
| Description: |
circ_BMPR2 is significantly up-regulated in ovarian granulosa cells from PCOS patients. It promotes KGN granulosa cell proliferation and suppresses apoptosis by acting as a ceRNA for miR-619-5p to regulate Rab43 expression. The study proposes circ_BMPR2 as a potential early diagnostic biomarker and mechanistic contributor to PCOS progression. |
| Confidence score: |
0.8455 |