| Expression pattern: |
UP |
| Associated gene: |
PDPK1, AGO2, AKT1, mTOR |
| Associated microRNA: |
miR-4725-5p |
| Biological function: |
Promotes ICC proliferation, invasion, migration, tumor growth, metastasis and gemcitabine resistance, while inhibiting apoptosis. |
| Molecular mechanism: |
circ_0084927 acts as a ceRNA sponge for miR-4725-5p to upregulate PDPK1 and activate the AKT/mTOR signaling pathway; exosome-mediated transfer of circ_0084927 confers gemcitabine resistance. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); drug resistance (promotes); chemoresistance (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; circRNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0084927 expression is associated with increased tumor number, advanced TNM stage, shorter OS and DFS, and is an independent risk factor for lower survival and recurrence in ICC patients. |
| Description: |
circ_0084927 is upregulated in ICC tissues and cell lines and high expression predicts poorer prognosis. It promotes ICC proliferation, invasion, migration, metastasis and tumor growth while inhibiting apoptosis through the miR-4725-5p/PDPK1/AKT/mTOR axis. It is also packaged into exosomes from gemcitabine-resistant ICC cells and transfers gemcitabine resistance to sensitive cells. |
| Confidence score: |
0.8884 |