| Expression pattern: |
UP |
| Associated gene: |
NRAS |
| Associated microRNA: |
miR-892a |
| Biological function: |
Promotes NSCLC cell proliferation, viability, migration, invasion, EMT, and tumor growth in vivo; knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
circ_0006528 acts as a miR-892a sponge and positively regulates NRAS expression, forming a circ_0006528/miR-892a/NRAS regulatory mechanism in NSCLC. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; MTT; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0006528 expression is associated with short survival time, tumor node and metastasis stage, and lymph node metastasis in NSCLC patients. |
| Description: |
circ_0006528 is up-regulated in NSCLC tissues and cell lines and high expression is associated with poor prognosis. Functionally, circ_0006528 promotes NSCLC proliferation, migration, invasion, EMT, and xenograft tumor growth. Mechanistically, it sponges miR-892a to positively regulate NRAS expression. |
| Confidence score: |
0.859 |