| Expression pattern: |
UP |
| Associated gene: |
G3BP1, BTRC |
| Associated microRNA: |
- |
| Biological function: |
Promotes gastric cancer cell proliferation, migration, invasion, EMT, tumorigenesis, tumor growth and metastasis in vitro and in vivo. |
| Molecular mechanism: |
hsa_circ_0000479 binds G3BP1 and stabilizes G3BP1 protein by inhibiting BTRC-mediated ubiquitination-mediated degradation through the ubiquitin-proteasome pathway. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ubiquitination (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Upregulated hsa_circ_0000479 expression in GC tissues was associated with poor clinical prognosis and was identified as a potential biomarker and therapeutic target for gastric cancer. |
| Description: |
hsa_circ_0000479/circEPSTI1 is an upregulated cytoplasmic circRNA in gastric cancer that promotes malignant progression, including proliferation, migration, EMT, tumor growth and metastasis. Mechanistically, it interacts with G3BP1 and blocks BTRC-mediated ubiquitination-dependent degradation of G3BP1, supporting the hsa_circ_0000479-G3BP1-BTRC axis as a potential therapeutic target. |
| Confidence score: |
0.8661 |