circRNA basic information
circBase ID: hsa_circ_0074158
Name: hsa_circ_CTNNA1
Synonym: circ_0074158
Host Gene: CTNNA1
Genomic location(hg19): chr5:138117611-138119061:+
Genomic location(hg38): chr5:138781922-138783372:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

subcutaneous tumour tissues
Cell lines:

A549; 293 T; LLC
In vivo animal model:

syngeneic model
circRNA-disease information
Expression pattern:
UN
Associated gene: QKI6, PD-L1
Associated microRNA: -
Biological function: Inhibits NSCLC cell proliferation, invasion and migration; activates CD8+ T cells and inhibits immune escape; promotes tumour cell apoptosis; suppresses tumour growth.
Molecular mechanism: circ_0074158 binds to the RBP QKI6, upregulates QKI6, and enhances PD-L1 ubiquitination, reducing PD-L1 protein levels and limiting immune evasion.
Biological pathway or process:

ubiquitination (promotes); immune regulation (promotes); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes)
Detected method:
Q
S
Validation methods:

circRNA-seq; RIP (RNA Immunoprecipitation); RT-qPCR; Western Blot; RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; ELISA; TUNEL; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis
Clinical significance:

-
Description:

In NSCLC models, hsa_circ_0074158 binds the RBP QKI6 and increases PD-L1 ubiquitination, lowering PD-L1 protein and reducing immune evasion. Functionally, circ_0074158 suppresses proliferation/invasion/migration and enhances CD8+ T-cell activity, promoting tumour cell apoptosis and inhibiting tumour growth.
Confidence score:

0.7878
Other information
Title:

Circ_0074158 and QKI6: A regulatory axis for PD-L1 ubiquitination and immune evasion in NSCLC.

Journal: Cancer immunology, immunotherapy : CII
Published: 2025
PubMed ID: 40931215
Study type:

combined biological and clinical study
Data availability: The data supporting the findings of this study are included within the article.
Code availability: -