| Expression pattern: |
UP |
| Associated gene: |
LIF, STAT3, AGO2 |
| Associated microRNA: |
miR-140-3p, miR-335 |
| Biological function: |
promotes proliferation, migration and invasion; promotes growth and metastasis |
| Molecular mechanism: |
ceRNA sponging of miR-140-3p and miR-335 to regulate LIF and activate LIF-STAT3 signaling |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); JAK/STAT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; EdU Staining; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circSCAF8 expression in PCa tissues was a powerful predictor of progression-free survival (HR = 2.14, P = 0.022). |
| Description: |
circSCAF8 is up-regulated in prostate cancer tissues and urine extracellular vesicles and promotes PCa cell proliferation, migration, invasion, and in vivo growth/metastasis. It acts as a ceRNA sponging miR-140-3p and miR-335 to upregulate LIF and activate the LIF-STAT3 pathway, and high circSCAF8 expression predicts poorer progression-free survival. |
| Confidence score: |
0.8868 |