| Expression pattern: |
UP |
| Associated gene: |
EIF4A3 |
| Associated microRNA: |
- |
| Biological function: |
Promotes HepG2 cell proliferation, migration and invasion; promotes HCC progression in vitro and tumor growth in vivo depending on EIF4A3. |
| Molecular mechanism: |
circRSF1 interacts with EIF4A3, and EIF4A3 enhances circRSF1 stability; circRSF1 depends on EIF4A3 to maintain its stability and promote malignant phenotypes. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
Elevated circRSF1 levels were observed in clinical HCC samples, and circRSF1 was suggested as a potential therapeutic target. |
| Description: |
circRSF1 (hsa_circ_0096491) is up-regulated in HCC tissues and IL-1beta-treated HepG2 cells. It promotes HepG2 proliferation, migration, invasion and xenograft tumor growth by interacting with the RNA-binding protein EIF4A3, which stabilizes circRSF1. The study suggests circRSF1 may act as an oncogenic mediator of inflammation-associated HCC progression and a potential therapeutic target. |
| Confidence score: |
0.8107 |