| Expression pattern: |
UP |
| Associated gene: |
RGS12, QKI, CTNNA1, GSK-3b |
| Associated microRNA: |
hsa-miR-1197, hsa-miR-1248, hsa-miR-1231, hsa-miR-1286 |
| Biological function: |
Potential biomarker for HCC diagnosis and monitoring recurrence; contributes to occurrence and development of liver carcinoma (as inferred by pathway/functional analyses). |
| Molecular mechanism: |
ceRNA network with predicted miRNAs and downstream target genes; circRNA-miRNA-mRNA regulatory network construction. |
| Biological pathway or process: |
ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Diagnostic biomarker for HCC; plasma/serum levels decrease after surgery and increase with recurrence; ROC AUC reported and combined diagnosis with AFP or RGS12 improves sensitivity and specificity. |
| Description: |
hsa_circ_0006091 is up-regulated in HCC tissues and is detectable in serum, showing postoperative decline and recurrence-associated rebound. The study constructs a predicted circRNA-miRNA-mRNA network (4 miRNAs) and supports its use as a diagnostic/monitoring biomarker, with improved ROC performance when combined with AFP or RGS12. |
| Confidence score: |
0.6957 |