| Expression pattern: |
UP |
| Associated gene: |
Wnt2B, beta-catenin, bax, bcl-2 |
| Associated microRNA: |
miR-4491 |
| Biological function: |
circ-BIRC6 promotes NSCLC cell proliferation, colony formation, migration, invasion, and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circ-BIRC6 acts as a molecular sponge for miR-4491 and negatively regulates miR-4491, thereby involving the Wnt2B/beta-catenin signaling pathway and apoptosis-related proteins. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; TUNEL; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ-BIRC6 might be a potential target for treatment of NSCLC. |
| Description: |
circ-BIRC6 is upregulated in NSCLC tissues and cell lines. Its knockdown suppresses proliferation, colony formation, migration, invasion, and xenograft tumor growth while promoting apoptosis. Mechanistically, circ-BIRC6 sponges and negatively regulates miR-4491 and is linked to activation of the Wnt2B/beta-catenin pathway, suggesting therapeutic potential in NSCLC. |
| Confidence score: |
0.6544 |