circRNA basic information
circBase ID: hsa_circ_0102543
Name: hsa_circ_DPF3
Synonym: circ_0102543 / cricDPF3
Host Gene: DPF3
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
684, 686
Disease MeSH ID:
D006528
Disease NCIt ID:
C3099
Disease ICD11 ID:
1294035808
Disease OMIM ID:
114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumors; adjacent normal tissues

Cell lines:

THLE-2; Huh-7; MHCC97H; MHCC97L; SNU449

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: SGTB, Ago2
Associated microRNA: miR-942-5p
Biological function: Suppresses proliferation, migration and invasion; promotes apoptosis; inhibits tumor growth in vivo
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-942-5p to regulate SGTB expression
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Sanger Sequencing; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; MTT; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; IHC (Immunohistochemistry); Clinical Sample Validation; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis

Clinical significance:

Low expression level of circ_0102543 was significantly correlated with the advance TNM stage, lymph node metastasis, and fibrosis; the overall survival rate of the high circ_0102543 expression group was significantly higher than that of the group with low circ_0102543 expression.

Description:

circ_0102543 is down-regulated in HCC tissues/cell lines and functions as a tumor suppressor. It sponges miR-942-5p to de-repress SGTB, thereby inhibiting proliferation, migration, and invasion while promoting apoptosis, and it reduces xenograft tumor growth.

Confidence score:

0.8465

Other information
Title:

Circ_0102543 suppresses hepatocellular carcinoma progression through the miR-942-5p/SGTB axis.

Journal: Annals of gastroenterological surgery
Published: 2023
PubMed ID: 37416745
Study type:

combined biological and clinical study

Data availability: The datasets used and/or analyzed during the current study are available within the manuscript and its supplementary information files.
Code availability: -