| Expression pattern: |
DN |
| Associated gene: |
DHX15, NF-kappaB p65 |
| Associated microRNA: |
miR-934, miR-942-5p |
| Biological function: |
inhibits proliferation and migration; suppresses tumor growth in vivo |
| Molecular mechanism: |
circRNF10 binds DHX15 in the cytoplasm and competitively impairs DHX15-p65 interaction, inhibiting NF-kappaB activation and antagonizing the DHX15-p65 positive feedback loop |
| Biological pathway or process: |
NF-kappaB (inhibits); proliferation (inhibits); migration (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; IF (Immunofluorescence); Co-IP; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; MTT; Colony Formation Assay; Wound Healing Assay; Transwell Assay; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Clinical Sample Validation; RNA-seq |
| Clinical significance: |
lower expression of circRNF10 was related to poor prognosis of patients with BC; lower circRNF10 expression led to shorter PFS |
| Description: |
circRNF10 is down-regulated in breast cancer and acts as a tumor suppressor. It binds the RBP DHX15 in the cytoplasm, competitively weakening DHX15-NF-kappaB p65 interaction to inhibit NF-kappaB signaling and disrupt the DHX15-p65 positive feedback loop, thereby reducing proliferation/migration and suppressing xenograft growth. |
| Confidence score: |
0.8988 |