| Expression pattern: |
UP |
| Associated gene: |
FOXK1, AGO2 |
| Associated microRNA: |
miR-646 |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, tumor growth and lung metastasis; reduces reactive oxygen species generation. |
| Molecular mechanism: |
circPPP2R4 acts as a ceRNA and miRNA sponge for miR-646, upregulates FOXK1 expression, and reduces ROS production to promote CRC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Potential diagnostic biomarker; high circPPP2R4 levels were positively correlated with advanced clinical stage, T stage and lymph node metastasis. |
| Description: |
circPPP2R4 is upregulated in colorectal cancer tissues and cells and is associated with advanced clinical stage, T stage and lymph node metastasis. It promotes CRC proliferation, migration, invasion, tumor growth and lung metastasis by acting as a miR-646 sponge to upregulate FOXK1 and reduce ROS production. The study suggests circPPP2R4 has potential diagnostic and therapeutic value in CRC. |
| Confidence score: |
0.8933 |