| Expression pattern: |
UP |
| Associated gene: |
HNF1beta, Derlin-1, Argonaute2 |
| Associated microRNA: |
miR-217 |
| Biological function: |
promotes cell proliferation, migration, and invasion; inhibits apoptosis; promotes glioma malignant progression |
| Molecular mechanism: |
Acts as a miR-217 sponge and forms an Argonaute2-dependent reciprocal negative feedback with miR-217, thereby regulating HNF1beta and downstream Derlin-1, involving PI3K/AKT and ERK signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); PI3K/AKT (promotes); ERK (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
FISH / smFISH; RT-qPCR; RNase R Treatment; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Circ-TTBK2 expression was positively correlated with the pathological grades of glioma. |
| Description: |
circ-TTBK2 is up-regulated in glioma tissues/cells and promotes malignant phenotypes (proliferation, migration, invasion) while inhibiting apoptosis. Mechanistically, it sponges miR-217 in an Ago2/RISC-dependent manner, thereby derepressing the miR-217 target HNF1beta and its downstream effector Derlin-1, with involvement of PI3K/AKT and ERK signaling and in vivo tumor growth effects. |
| Confidence score: |
0.817 |