| Expression pattern: |
DN |
| Associated gene: |
TJP1, CDH2, VIM |
| Associated microRNA: |
hsa-miR-24-3p, hsa-miR-138-5p, hsa-miR-145-3p, hsa-miR-620, hsa-miR-875-3p |
| Biological function: |
Suppresses HCC cell migration and invasion; induces cell cycle arrest (G2/M) without significantly affecting apoptosis; encodes a protein. |
| Molecular mechanism: |
May act through a ceRNA network (circEPS15-miRNA-mRNA); modulates TJP1/CDH2/VIM signaling; contains a spanning junction ORF with IRES-driven translation to produce a novel protein. |
| Biological pathway or process: |
Notch (other); inflammation (other); cell cycle (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; ROC Analysis; Survival Analysis; Transfection; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
Low circEPS15 levels were significantly associated with poor overall survival; circEPS15 showed diagnostic significance for HCC (AUC = 0.735). |
| Description: |
circEPS15 (circRNA-100226) is down-regulated in HCC tissues and low expression is associated with poor overall survival. circEPS15 overexpression inhibits HCC cell migration/invasion and induces G2/M cell-cycle arrest, potentially via a ceRNA network and modulation of TJP1/CDH2/VIM signaling. It also contains an IRES-driven spanning-junction ORF that can be translated into a novel protein. |
| Confidence score: |
0.835 |