| Expression pattern: |
UP |
| Associated gene: |
alpha-Syn, p62, SQSTM1/p62 |
| Associated microRNA: |
miR-588 |
| Biological function: |
Promotes alpha-Syn expression, accumulation and aggregation; suppresses autophagic clearance through the miR-588/p62 autophagy pathway. |
| Molecular mechanism: |
circ_0005654 acts as a molecular sponge for miR-588, enhancing alpha-Syn RNA/protein expression and regulating alpha-Syn through the miR-588/p62 autophagy pathway. |
| Biological pathway or process: |
ceRNA regulation (promotes); autophagy (inhibits); other pathway/process (promotes) |
| Detected method: |
S
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0005654 showed diagnostic potential for PD and was proposed as a promising therapeutic candidate for targeted modulation of alpha-Syn aggregation. |
| Description: |
circ_0005654 is up-regulated in peripheral blood of PD patients and is derived from the PRDM5 gene. In cellular PD models, it acts as a miR-588 sponge to promote alpha-Syn mRNA/protein expression and suppress alpha-Syn clearance through the miR-588/p62 autophagy pathway, contributing to alpha-Syn accumulation and aggregation. The study suggests circ_0005654 has diagnostic potential and may be a therapeutic target for PD. |
| Confidence score: |
0.8193 |