| Expression pattern: |
UP |
| Associated gene: |
MAPK14 |
| Associated microRNA: |
miR-375 |
| Biological function: |
circ_0008285 promotes LPS-induced macrophage inflammatory polarization, TC-1 cell injury and apoptosis, and lung injury in sepsis-induced ALI; knockdown of circ_0008285 reduces inflammatory cytokines, shifts macrophage markers toward M2 polarization, improves TC-1 viability, decreases apoptosis, and alleviates lung damage in CLP mice. |
| Molecular mechanism: |
circ_0008285 acts as a miR-375 sponge and regulates MAPK14 through the miR-375/MAPK14 axis, thereby affecting macrophage polarization, inflammatory injury and apoptosis in sepsis-induced ALI. |
| Biological pathway or process: |
MAPK (promotes); ceRNA regulation (other); immune regulation (promotes); macrophage polarization (other); inflammation (promotes); apoptosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Annexin V/PI Flow Cytometry; In Vivo Animal Model; H&E Staining; TUNEL |
| Clinical significance: |
Circ_0008285 may be served as a potential therapeutic object for sepsis-induced ALI. |
| Description: |
This study found that circ_0008285 is up-regulated in LPS-treated macrophages and CLP-induced sepsis-ALI mouse lung tissue. Functionally, circ_0008285 promotes inflammatory macrophage polarization, epithelial cell injury/apoptosis, and lung damage, while its knockdown alleviates these phenotypes. Mechanistically, circ_0008285 acts through the miR-375/MAPK14 axis and may represent a therapeutic target for sepsis-induced ALI. |
| Confidence score: |
0.5634 |