| Expression pattern: |
UP |
| Associated gene: |
Wnt3 |
| Associated microRNA: |
miR-424-5p |
| Biological function: |
Promotes clone formation, inhibits apoptosis, reduces temozolomide sensitivity, promotes tumor growth, and contributes to TMZ resistance in glioma cells. |
| Molecular mechanism: |
circ_EPHB4 functions as a molecular sponge for miR-424-5p, relieving miR-424-5p-mediated suppression of Wnt3 and forming a circRNA-miRNA-mRNA ceRNA regulatory axis in glioma TMZ resistance. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circ_EPHB4 is a potential target for reversing glioma drug resistance and overcoming glioma chemoresistance. |
| Description: |
circ_EPHB4 is up-regulated in TMZ-resistant glioma tissues and cells. It promotes clonogenic growth and chemoresistance while inhibiting apoptosis by sponging miR-424-5p and increasing Wnt3 expression. Knockdown of circ_EPHB4 enhances TMZ sensitivity in vitro and suppresses xenograft tumor growth in vivo. |
| Confidence score: |
0.7045 |