| Expression pattern: |
DN |
| Associated gene: |
IGF2BP3 |
| Associated microRNA: |
- |
| Biological function: |
circPLEKHH2 acts as a tumor suppressor in LUAD by promoting autophagy and inhibiting LUAD cell proliferation, invasion, migration, and tumor growth. |
| Molecular mechanism: |
IGF2BP3 directly binds to circPLEKHH2 and inhibits its expression; circPLEKHH2 promotes autophagy and suppresses LUAD progression through inhibition of the AKT/mTOR signaling pathway. |
| Biological pathway or process: |
autophagy (promotes); PI3K/AKT/mTOR (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); IHC (Immunohistochemistry); In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circPLEKHH2 expression in patient tissue has potential diagnostic value to distinguish LUAD tissue from normal lung tissue; ROC AUC was 0.949. |
| Description: |
circPLEKHH2 is significantly downregulated in LUAD and functions as a tumor suppressor. It promotes autophagy and suppresses LUAD cell proliferation, migration, invasion, and xenograft tumor growth through inhibition of the AKT/mTOR signaling pathway. IGF2BP3 directly binds circPLEKHH2 and inhibits its expression, which may contribute to circPLEKHH2 downregulation in LUAD. |
| Confidence score: |
0.8554 |