| Expression pattern: |
UP |
| Associated gene: |
ETS1, ACAN, COL2A1 |
| Associated microRNA: |
- |
| Biological function: |
circETS1 is associated with IDD progression; inhibition of circETS1 enhances NPC viability and proliferation, mitigates TNF-alpha-induced apoptosis, promotes extracellular matrix secretion and repair, reduces inflammation, and delays IDD progression. |
| Molecular mechanism: |
Silencing circETS1 using si-circETS1 delivered by PLGA microspheres suppresses circETS1 expression without impacting linear ETS1 mRNA, thereby improving NPC activity and extracellular matrix metabolism in IDD models. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; Transfection; Annexin V/PI Flow Cytometry; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circETS1 could serve as an effective therapeutic target for IDD, and PLGA microspheres loaded with si-circETS1 represent a promising therapeutic strategy. |
| Description: |
This study identified hsa_circ_0002083/circETS1 as an ETS1-derived circRNA up-regulated in severely degenerated human nucleus pulposus tissues and TNF-alpha-induced degenerative NPCs. Functionally, circETS1 appears to aggravate IDD-related NPC dysfunction by suppressing proliferation, promoting apoptosis, and impairing extracellular matrix metabolism, while si-circETS1 delivery using PLGA microspheres suppresses circETS1 and delays IDD progression in vitro and in vivo. |
| Confidence score: |
0.8037 |