circRNA basic information
circBase ID: hsa_circ_0001136
Name: hsa_circ_ASXL1
Synonym: circASXL1
Host Gene: ASXL1
Genomic location(hg19): chr20:30954186-30956926:+
Genomic location(hg38): chr20:32366383-32369123:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021178
MONDO name: injury
Disease details: arsenic-induced pulmonary pathologies
Disease DO ID:
 -
Disease MeSH ID:
 D014947
Disease NCIt ID:
 C3671
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

16HBE
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: STAT3, P65, ILF3, IKBA, P50, PCNA, BCL2, BCL-XL, CIAP1, Cyclin A2, CDK2
Associated microRNA: -
Biological function: circASXL1 knockdown enhanced 16HBE cell viability and proliferation; arsenic-induced circASXL1 may influence proliferation and apoptosis of bronchial epithelial cells through the P65 pathway.
Molecular mechanism: Arsenic modulates circASXL1 to engage STAT3-P65 crosstalk; circASXL1 knockdown activates STAT3 to promote P65 transcription and NF-kappaB pathway-related proliferative signaling, with ILF3 contributing to circASXL1 production.
Biological pathway or process:

NF-kappaB (inhibits); JAK/STAT (inhibits); proliferation (inhibits); apoptosis (other); ubiquitination (other)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Western Blot; Co-IP
Clinical significance:

-
Description:

In 16HBE human bronchial epithelial cells, sodium arsenite increased circASXL1 expression. Functional knockdown experiments showed that reduced circASXL1 promotes cell viability and proliferation, associated with STAT3 activation, increased P65 transcription, NF-kappaB pathway activation, and altered P65 ubiquitination. ILF3 knockdown reduced circASXL1 expression, suggesting ILF3 may regulate circASXL1 biogenesis in this arsenic-induced lung toxicity model.
Confidence score:

0.5554
Other information
Title:

Arsenic-induced circASXL1 regulates 16HBE cell proliferation through the P65 signaling axis.

Journal: Ecotoxicology and environmental safety
Published: 2025
PubMed ID: 40349468
Study type:

biological research
Data availability: Data will be made available on request.
Code availability: -