| Expression pattern: |
UP |
| Associated gene: |
p-Akt, Rb, p-Rb, E2F1, Akt |
| Associated microRNA: |
- |
| Biological function: |
promotes cell proliferation and regulates G1-S transition in ERMS-derived cells |
| Molecular mechanism: |
counteracts p-Akt proteasome-dependent degradation and affects pRb/Rb ratio |
| Biological pathway or process: |
cell cycle (other); proliferation (promotes); PI3K/AKT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNA-seq; FISH / smFISH; Transfection; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; Bioinformatics Analysis; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circ-ZNF609 is up-regulated in RMS (ERMS and ARMS) tumors and cell lines. In ERMS-derived RD cells, circ-ZNF609 knockdown induces a G1/S arrest and reduces p-Akt and pRb/Rb, with evidence that it counteracts proteasome-dependent degradation of p-Akt, supporting a role in promoting proliferation via PI3K/AKT-related cell-cycle control. |
| Confidence score: |
0.6221 |