circRNA basic information
circBase ID: hsa_circ_0005328
Name: hsa_circ_QKI
Synonym: circQKI
Host Gene: QKI
Genomic location(hg19): chr6:163876310-163899928:+
Genomic location(hg38): chr6:163455278-163478896:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

subcutaneous tumors; xenograft tumor tissues
Cell lines:

PC3; PC3/DR; 22RV1; 22RV1/DR
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: Beclin-1, METTL3, IGF2BP2, Ago2
Associated microRNA: miR-188-3p
Biological function: promotes docetaxel resistance, activates autophagy, enhances Beclin-1 expression, reduces docetaxel-induced apoptosis, and promotes colony formation/proliferative survival under docetaxel treatment
Molecular mechanism: METTL3-mediated m6A modification stabilizes circQKI through IGF2BP2 recognition; accumulated circQKI acts as a ceRNA sponge for miR-188-3p, derepresses Beclin-1, activates autophagy, and drives docetaxel resistance
Biological pathway or process:

autophagy (promotes); apoptosis (inhibits); proliferation (promotes); chemoresistance (promotes); drug resistance (promotes); ceRNA regulation (other); m6A modification (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; TUNEL; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

-
Description:

circQKI is upregulated in docetaxel-resistant prostate cancer cells and reduces docetaxel sensitivity. METTL3-mediated m6A modification stabilizes circQKI through IGF2BP2, and accumulated circQKI sponges miR-188-3p to increase Beclin-1 expression, activate autophagy, inhibit apoptosis, and promote chemoresistance.
Confidence score:

0.86
Other information
Title:

N6-methyladenosine-modified circQKI inhibits prostate cancer docetaxel-sensitivity via miR-188-3p/Beclin-1 pathway.

Journal: Life sciences
Published: 2025
PubMed ID: 40250752
Study type:

biological research
Data availability: All data included in this study are available upon request by contact with the corresponding author; Supplementary data to this article can be found online at https://doi. org/10.1016/j.lfs.2025.123646.
Code availability: -