circRNA basic information
circBase ID: hsa_circ_0031250
Name: hsa_circ_PRMT5
Synonym: Circular RNA 0031250
Host Gene: PRMT5
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small-cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

chemoresistant NSCLC tissues; sensitive specimens; excised tumors

Cell lines:

A549; H460; A549/DDP; H460/DDP

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: REV3L, P-gp, GST-pi
Associated microRNA: miR-4458
Biological function: circ-PRMT5 promotes DDP resistance, cell viability, invasion, metastasis-related phenotypes, and tumor growth, while suppressing apoptosis and DDP sensitivity in DDP-resistant NSCLC.
Molecular mechanism: circ-PRMT5 functions as a ceRNA by sponging miR-4458 to regulate REV3L, thereby promoting cisplatin resistance in NSCLC.
Biological pathway or process:

apoptosis (inhibits); invasion (promotes); metastasis (promotes); chemoresistance (promotes); drug resistance (promotes); ceRNA regulation (other)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model

Clinical significance:

circ-PRMT5 may provide a novel therapeutic strategy and might serve as a novel thought in the diagnosis and prognosis of NSCLC.

Description:

This study found that circ-PRMT5 is up-regulated in cisplatin-resistant NSCLC tissues and cells. Functionally, circ-PRMT5 promotes DDP resistance, invasion, and tumor growth and inhibits apoptosis by acting as a sponge for miR-4458 to up-regulate REV3L.

Confidence score:

0.7177

Other information
Title:

Circular RNA PRMT5 confers cisplatin-resistance via miR-4458/REV3L axis in non-small-cell lung cancer.

Journal: Cell biology international
Published: 2020
PubMed ID: 32808744
Study type:

combined biological and clinical study

Data availability: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Code availability: -