| Expression pattern: |
UP |
| Associated gene: |
TLR-4, NF-kappaB, TLR4, IkappaBalpha, p65 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Circ_0001715 promotes proliferation and inflammation and suppresses apoptosis in RA fibroblast-like synoviocytes; knockdown of circ_0001715 decreases proliferation and inflammation, promotes apoptosis, and mitigates pathological manifestations in the CIA mouse model. |
| Molecular mechanism: |
Circ_0001715 acts through a ceRNA mechanism by sequestering miR-326, thereby regulating the TLR-4/NF-kappaB axis in RA fibroblast-like synoviocytes. |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); apoptosis (inhibits); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
Circ_0001715 may be a target for the diagnosis and treatment of RA. |
| Description: |
Circ_0001715 is up-regulated in RA patient synovial tissues, TNF-alpha-induced HFLS-RA cells, and CIA mouse synovial tissues. Functionally, circ_0001715 promotes proliferation and inflammation and inhibits apoptosis in RA-FLSs. Mechanistically, it sequesters miR-326 to regulate the TLR-4/NF-kappaB axis, suggesting potential diagnostic and therapeutic value in RA. |
| Confidence score: |
0.6835 |