circRNA basic information
circBase ID: hsa_circ_0000247
Name: hsa_circ_HMCU
Synonym: circHMCU
Host Gene: HMCU
Genomic location(hg19): chr10:74474868-74475660:+
Genomic location(hg38): chr10:72715110-72715902:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissue samples; adjacent normal tissue samples; tumor tissues; normal tissues
Cell lines:

MDA-MB-231; MCF-7; 293 T; MCF10A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PGK1, AGO2
Associated microRNA: miR-4458
Biological function: promotes cell proliferation, migration, invasion, glycolysis and tumor growth; inhibits apoptosis in breast cancer
Molecular mechanism: Acts as a miRNA sponge for miR-4458 to upregulate PGK1 expression (ceRNA-like regulation), promoting malignant phenotypes and glycolysis in breast cancer.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circHMCU might be an underlying prognostic biomarker for BC.
Description:

circHMCU (hsa_circ_0000247) is up-regulated in breast cancer tissues and cell lines and acts as an oncogenic circRNA. It sponges miR-4458 to increase PGK1, thereby promoting glycolysis and malignant phenotypes (proliferation, migration/invasion) and suppressing apoptosis, and its knockdown reduces xenograft tumor growth.
Confidence score:

0.8299
Other information
Title:

Circular RNA circHMCU promotes breast tumorigenesis through miR-4458/PGK1 regulatory cascade.

Journal: Hereditas
Published: 2023
PubMed ID: 36949536
Study type:

combined biological and clinical study
Data availability: The online version contains supplementary material available at 10.1186/s41065-023-00275-y.
Code availability: -