| Expression pattern: |
UN |
| Associated gene: |
EML4-ALK1, SMAD4, ADAR1 |
| Associated microRNA: |
miR-4673 |
| Biological function: |
F-circEA1 promotes proliferation, migration, invasion and xenograft tumor growth of EML4-ALK1-positive LUAD cells; interference with F-circEA1 inhibits xenograft growth and EML4-ALK1 expression. |
| Molecular mechanism: |
F-circEA1 sponges miR-4673 and participates in a feedback pathway involving SMAD4 and ADAR1 to regulate EML4-ALK1 expression; ADAR1 binds F-circEA1 and facilitates its expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other); TGF-beta/SMAD (other) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; IHC (Immunohistochemistry); ChIP / ChIP-seq; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
F-circEA1/miR-4673/SMAD4/ADAR1 pathway may provide therapeutic ideas and possible therapeutic direction for EML4-ALK1-positive LUAD. |
| Description: |
F-circEA1 is a human fusion circRNA associated with EML4-ALK1-positive lung adenocarcinoma. It promotes malignant phenotypes of LUAD cells by sponging miR-4673 and participating in a feedback network involving SMAD4, ADAR1 and EML4-ALK1; F-circEA1 interference suppresses xenograft growth and EML4-ALK1 expression. |
| Confidence score: |
0.7629 |