| Expression pattern: |
DN |
| Associated gene: |
PAPPA protein |
| Associated microRNA: |
- |
| Biological function: |
promotes trophoblast proliferation in vitro; influences trophoblast development |
| Molecular mechanism: |
circPAPPA binds PAPPA protein and promotes JAR cell growth by forming a circRNA-protein complex with PAPPA protein |
| Biological pathway or process: |
proliferation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
potential predictive disease target or therapeutic target; targeting the circPAPPA-PAPPA interaction might be a novel therapeutic strategy for addressing spontaneous abortion |
| Description: |
circPAPPA (hsa_circ_0003076), derived from the PAPPA gene, is down-regulated in villus tissues from spontaneous abortion. It promotes trophoblast/JAR cell proliferation in vitro by interacting with PAPPA protein and forming a circPAPPA-PAPPA circRNA-protein complex, suggesting potential therapeutic relevance in spontaneous abortion. |
| Confidence score: |
0.8311 |